Investigating Enzyme Activity :: Papers

Investigating Enzyme use PLANNING AIM === The aim of this experiment is to see how changing the slow-wittedness of hydrogen peroxide (H2O2) affects the rate of reaction of the enzyme Catalase. The reaction I am looking at is the breakdown of Hydrogen Peroxide into water and type O by the enzyme Catalase. The equation for this reaction is 2H2O2 2H2O + O2 FACTORS ------- In this experiment I could convince the following factors Concentration of the Hydrogen Peroxide or Catalase, the time, the temperature and the Ph of the solutions. scientific KNOWLEDGE (See appendix 1) PREDICTION ---------- My prediction is that the higher the concentration of Hydrogen Peroxide, the greater the amount of oxygen given off pass on be within a period of time. The lower the concentration of Hydrogen Peroxide the amount of oxygen given off go forth be little than if it was at a higher concentration. I think this because there are more particles in a substance to collide and react at a higher concentration. VARIABLES I am not going to modify anything other than the concentration of the Hydrogen Peroxide. The concentration of the Catalase enzyme, the temperature of the laboratory and the Ph of the solutions entrust remain constant. EQUIPMENT Hydrogen Peroxide (concentration - 0.5 1.0%, 1.5%, 2.0%, 2.5% and 3.0%) Safety Catalase Water trough, Burette, Stop clock, bringing vacuum tube, Clamp stand, Conical flask, Syringe, Pipette Glasses/goggles, PROCEDURE 1. I will take the equipment drawn and set it up as shown. *(appendix 2) 2. I will fill the burette and water trough with water. 3. Using a mensuration cylinder, I will put 10ml of Hydrogen Peroxide in the conical flask connected to the delivery tube. 4. Trying to prevent water from escaping, I shall turn the burette upside down and clamp it to the stand with the dirty dog of the burette in the water trough and feed the deliv ery tube through the burette.