The rate of catalase action Essay Example for Free

The locate of catalase work EssayI reach to develop goggles, prevent any accidents that can damage my eyes I break to make sure I handle all the glassware carefully I have to handle the atomic number 1 peroxide really carefully because it is in truth toxic Method 1. Set up the audition according to the diagram 2. nib 10 cm of catalase (celery juice) 3. Measure 10 cm of hydrogen Peroxide 4. Pour the catalase into the conical flask 5. Fill the burette with water 6. Pour the hydrogen peroxide into the conical flask and close the lean and start the durationr 7. Record the intensiveness of screw up devoted kill every 10 seconds ()8. Mix hydrogen peroxide with pure water to give the next parting of concentration of acid 100%, 80%, 60%, 40% and 20% 9. fictionalise 1 8 using the 100%, 80%, 60%, 40% and 20% hydrogen peroxide 10. Repeat the completely audition three times to get a more than good result 11. while graphs for each concentration -volume of gas i mperturbable against time 12. Draw best-fit pull ins Diagram Analysis After I have done the sample, I have got the results 1st result Time (Seconds) Volume of gas hoarded when there is 100% of H O (cm ).Volume of gas collected when there is 80% of H O (cm ) Volume of gas collected when there is 20% of H O (cm ) 1. 5 2. 6 3. 7 5. 0 6. 2 7. 5 8. 6 9. 6 Now I am press release to steep the graphs volume of gas collected against time. The graphs shows a curve best-fit line that means whenever the concentration augments, the volume of gas that was collected in the given time increases but the volume of gas collected increase at a slower rate at the end. The 100% concentration produced the closely oxygen in the shortest time, which gives it a higher reception rate than the others.According to the graph, it has proven my prediction is correct. The highest concentration would produce the most oxygen in the shortest time. The volume of gas collected increases at a slower rate at the end because the towards the end, there are less substrate and enzyme left, so it takes longer to react and releases the gas(oxygen). The rate of reaction is a measure of how fast the reaction takes place. A reaction will take place when the particles of the reacting substances jolt with each other and a fixed amount of energy called the activation energy is reached.If a encounter between particles can produce sufficient energy (i. e. if they collide fast enough and in the amend direction) a reaction will take place. This is called the Collision Theory for rates of reaction. Concentration of the substrate increases, the great the rate of reaction will be because higher concentration means a higher bend of particles in a given volume. If there are more particles, there will be more reaction. More reaction means a larger number of successful collisions. Therefore the catalase action rate will increase. Also, the particles are closer together, so they can collide more often.The more often they collide, the pretend of reaction happening increases. So the catalase action rate increases. Evaluation I think the results are very good that I can use them to support what I predicted at the beginning. I also have enough evidence to support my conclusion. The points on my graph are not very close to the best-fit line which is due to the large scale I used, so the gap seems to be bigger. But the points let me draw reasonable best-fit lines and they also show the pattern I expect. I think I can let off rely on the results because they show the general pattern for each concentration.I know my results are reliable because they fitted my prediction and it also enables me to explain the relation between concentration and rate of catalase reaction. Also, I repeated my experiment three times to obtain the results, so I know they are reliable. The appropriate apparatus I used, increases the accuracy of the results. I think my experiment is quite successful as I can collect re sults that help me to draw some accurate graphs except there are a few mistaken points. I think this happened because 1) I did not close the bung quick enough and some gas leak out which cause a decrease in the readings2) I did not use the like celery each time because 1 celery does not provide sufficient catalase for the whole experiment which can cause increase or decrease to the readings 3) The temperature is slightly incompatible as I did the experiment in three different days which can cause increase or decrease to the readings I think there is a better way of doing it that is to have a tap at the top. When I open it, the hydrogen peroxide will drop down into the catalase and I do not requirement to put the bung on because the bung is put on top of the tap which means no gas will leak out.Also, when I time it with a stopwatch, it will not be as accurate as using computers to time because human has reaction time that will increase the time taken and this will make the experi ment less accurate. I can do the experiment in this way For this experiment, I have used celery as a source of catalase, other than this I can use potatoes or liver that may affect the time taken to collect gas, as they are different. Further experiment Planning Apparatus A 50cm conical flask with a bung A burette Clamps and stand Delivery tube Container 10cm measuring cylinder A stopwatch. 20 vol of hydrogen peroxide Potato tubes (catalase) Safety control I have to make sure all the schooltime bags and chairs are under the table, in case I trip over I have to wear goggles, prevent any accidents that can damage my eyes I have to make sure I handle all the glassware carefully I have to handle the hydrogen peroxide very carefully because it is very toxic Method 1. Set up the experiment according to the diagram 2. Measure 10 g of catalase (potato juice) 3.Measure 10 cm of Hydrogen Peroxide 4. Pour the catalase into the conical flask 5. Fill the burette with water 6.Set up the light sensor and the computer 7. Turn on the tap and start the computer at the same time 8. Record the volume of gas given off every 10 seconds (10s, 20s, 30s, 40s, 50s, 60s, 70s and 80s) 9. Mix hydrogen peroxide with pure water to give the following percentage of concentration of acid 100%, 80%, 60%, 40% and 20% 10. Repeat 1 9 using the 100%, 80%, 60%, 40% and 20% hydrogen peroxide 11. Repeat the whole experiment three times to get a more accurate result 12. Plot graphs for different concentration- volume of gas collected against time 13. Draw best-fit lines 14.